Screening of phylloxera resistant grape rootstock (Ru140) at the nurseries of ministry of agriculture using SSR markers

Abstract

This investigation was conducted at the General Commission for Scientific Agricultural Researches, to screen the grape rootstock  (Ru140) as a resistant  rootstock to phylloxera in the germplasmes of the nurseries which belong to Ministry of Agriculture using 20 primer  pairs of SSR, the leaves samples were collected from 10 plants morphologically represent the rootstock (Ru140)  from each nursery; Babennes in Aleppo, Shaikh Hmaid in Homs and Kharabo in Damascus countryside, in the comparison with the positive control Ru140 which recently has introduced from Italy. The results showed the ability of 19 primer pairs to identify the samples which related to Ru140 rootstocks in each nursery, there were one different sample in Babennes nursery, four different samples in Shaikh Hmaid nursery and two different samples in Kharabo nursery, while the other samples in each nursery were identical to the positive control. According to these results, the irregular plants  in each nursery should be excluded. The allele size which represent each locus for all primers was identical to the expected size with the homologous plants, while it was variant in the dissenting plants. Since, SSR marker was an efficient tool to detect the genetic variation between individuals in the same species and distinguished the dissent plants out of Ru140 rootstock, which could be used as an efficient tool for screening and documentation rootstocks and cultivars in the germplasms at the nurseries in the integration with the availability of  positive controls.