Evalution mitochondrial DNA copy number and smoking risk of larynx squamous cell carcinoma in Syrian population

Abstract

Abstract:

Head and neck squamous cell carcinoma is the most commonly diagnosed cancer worldwide. The smoking habits, manifest the Syrian population toward higher susceptibility to develop head and neck squamous cell carcinoma. Here, we have investigated the association of smoking  with copy number variation of cell-free mitochondrial DNA in cases and controls groiups. Cell-free DNA from saliva was isolated from 64 larynx squamous cell carcinoma cases and 137 controls with informed written consent using QIAamp Circulating Nucleic Acid Kit. Real-time polymerase chain reaction was done for copy number variation in cell-free mitochondrial DNA. to evaluate the diagnostic application between the two study groups using clinicopathological parameters. Mann-Whitney U used to  measure the levels of cell-free mitochondrial DNA of cases in association with smoke  was significantly higher (p < 0.05) than controls. Using receiver operating characteristic curve (ROC) analysis between larynx squamous cell carcinoma cases and controls, we distinguished cell-free mitochondrial DNA (cutoff: 3.857.827; sensitivity: 0.422; specificity: 0.832;  p < 0.001)

Our result indicates that the cell-free mitochondrial DNA content is highly associated with smoking which shows greater promises, holding the key characteristics of diagnostic biomarkers, that is, minimal invasiveness, high specificity, and sensitivity.