Evaluation of the Photodynamic Therapy in The Treatment of the Denture Stomatitis

Abstract

Background: Denture stomatitis is a common condition in denture users, and photodynamic therapy (PDT) has been shown to be  effective  against candida albicans  in many studies.  This research aimed was to evaluate the effective of  photodynamic therapy PDT   with methylene blue MB  using Diode laser  in the treatment of denture stomatitis.

Materials and methods: The study sample consists of 11 patients (7males and 4 females) selected from the oral medicine clinic at Faculty of Dentistry, Damascus University, and the participants with clinical and microbiological diagnoses of type  ( I and II) denture stomatitis   received six sessions of PDT with MB (450µg/mL) , and after 10 min of incubation, were illuminated by light emitting-diode light at 650 nm   two times a week for 20 days. Mycological cultures taken from dentures and palates and standard photographs of the palates were taken at baseline (day 0), at the end of the treatment  and at the follow-up time intervals (days  15 , 30 and 60). The colony-forming units (CFU ) were  seeded and quantified on Sabouraud agar tubes. Data were analysed by paired-samples T test.

Results: The treatment significantly reduced the CFU  at the end of the treatments and on 15, 30 ,and 60 day of the follow-up period.  Also the   PDT  showed clinical success to relief the inflammation on the palates  and  decreased the oral lesion and clinical symptoms for the patients at the end of the treatments and on day 30 and 60 of the follow-up period. 

Conclusions: This clinical study suggests that PDT can reduce fungal load and improve the clinical symptoms in patients with denture stomatitis.