Effect of citric acid and EDTA 17% as chelating soluations on the microhardness of dentin- An in-vitro Study

Abstract

Backgrounds and Objectives: The irrgation is one of the most important procedures in endodontic treatments, as many clinical and laboratory studies have shown that mechanical preparation of root canals leaves large areas of canal walls not prepared. Therefore, it is necessary to combine mechanical and chemical preparation through irrgation solutions, despite the use of sodium hypochloride as a liquid irrgation is a major in endodontic treatments, but it is unable to be the only one used in endodontic treatment, due to its inability to remove the smear layer formed after preparing the root canal, so it was used to share it with chelating fluids the objective of this research is To investigate the effect of chelating fluids on the microhardness of dentin that affects the performance and durability of all materials used for root canal sealing.

Materials and Methods: The sample size was 36 single-root teeth distributed into four groups, 9 slides in each group. Each group was treated by immersion in a chelating fluid. Four chelating fluids were used: EDTA 17%, citric acid 10%, citric acid 40%. Each group was subjected to microhardness measurement ،Each group underwent a Vickers-based micro hardness measurement before and after the application of chelating fluids. Student's t-test was also used to study the differences in microhardness between the two stages before and after the application of lavage fluids in each group. One Way Anova test was used to study the differences in the microhardness of root dentin. After applying the washing liquids between groups of samples and to reveal the direction of the statistically significant differences between the groups, the (LSD) test was applied to identify the direction of the statistically significant differences.

Results: The differences in the microhardness of root dentin between groups of research samples compared to before and after washing the samples in each third of the teeth and in the total average ranged between (0.000-0.024), all of which are smaller than the significance level (0.05), and therefore there are statistically significant differences in the mean Measurements of the microhardness of root dentin between groups of research samples, and there are no statistically significant differences in the average amount of change in the microhardness of root dentin in the binary comparisons between groups of research samples.

Conclusions: Within the limits of this study, all chelating fluids can be  have reduced the microhardness of root dentin without having a statistically significant difference between them by affecting dentin.