A study of Ki-67 protein expression in the developmental stages of hamster-induced squamous cell carcinoma

Abstract

Background & Aim : That abnormal cell proliferation appears to be a  may be a predictor of tumor genesis, The Ki-67 protein is closely linked to cell proliferation and is used in pathogenesis as a marker to determine cell growth in human tumors The aim of this study to demonstrate the presence, location and pattern of cell proliferation in different histological grades of oral squamous cell carcinoma and normal oral epithelium (NOE) using an antibody directed against the Ki-67 antigen.

Materials & Methods:

The sample consisted of two groups, the first 20 hamsters with the carcinogenic substance DMBA applied in the cheek sinus, and the second 5 hamsters without applying any carcinogenic substance (control sample), and biopsies were taken from the carcinogenic cheek sinus of the first group at intervals and from the healthy cheek sinus of the second control group, which was stained Conventionally, hematoxylin-eosin and immunoglobulin by a polyclonal antibody to the Ki-67 cell division marker.

Results: Ki-67 labeling Index (LI) was restricted to the basal  of the normal oral epithelium whereas Ki-67 positive cells in oral epithelial dysplasia (OED) were located in the basal, suprabasal and spinous layers. Ki-67 LI is increased in high risk cases than the low risk cases of OED, Statistical analysis  indicate significant differences between the (Ki-67 LI) in grade II-III of (OED) and normal oral epithelium.

Conclusion: This study showed that presence, location expression  Ki-67 antibody in proliferating cell distribution in the layers of oral epithelial dysplasias may provide useful information to evaluate the grading of OED. Ki-67 LI increased in high risk cases than low risk cases of OED