Polymorphism -381A/G (rs1320632) of Collagenase (MMP-8) Gene Promoter and Periodontitis in Type II Diabetic Syrian Population

Abstract

Introduction: Matrix metalloproteinases-8 (MMP-8) is involved in a number of physiological events, and has a crucial role in collagen breakdown during periodontal tissue destruction. Diabetes mellitus (DM) has been associated with altered collagen metabolism. It increases the response of the periodontal tissue to pathogenic microorganisms, thereby increasing the severity of periodontal disease. The aim of this study is to investigate the association between (-381A/G) polymorphism in MMP-8 gene promoter and chronic periodontitis (ChP) in Syrian patients with type II diabetes mellitus (DM).

Received: 22/11/2021

Accepted: 24/2/2022

Copyright: Damascus University- Syria, The authors retain the copyright under a  CC BY- NC-SA 

Materials and Methods: The study is designed as a cross-sectional study, consists of 180 participants distributed into four groups (ChP, DM, ChP with DM (ChPDM) and healthy controls. Genomic DNA has been extracted from the whole blood collected from the participants. DNA was amplified using specific primers flanking -381A/G point in MMP-8 gene promoter. The genotyping was performed by restriction fragment length polymorphism (RFLP) using MboI enzyme. The distribution of genotypes and allele frequencies between the study groups was compared using the Chi-squared test.

 Results: The genotype frequencies showed significant differences in AA genotype frequency between each patient group and healthy group (P˂ 0.05), and subjects carrying the G allele were significantly more likely to develop ChP with DM (OR= 2.41, CI: 0.998-1.019, P = 0.048) and without DM (OR= 6, CI: 1.78-20.147, P = 0.002) compared with C subjects.

Conclusion: The present study suggests that the MMP8 (-381A/G) polymorphisms has an important role in periodontitis susceptibility in Syrian diabetic population.