Depth and spread burn healing by using dried and gamma irradiation sterilized amniotic membrane (AmnioGraft)

Abstract

Abstract:

Human amniotic membrane (HAM) has been used as skin-graft for reconstructing of damaging inner/external tissues, and in plastic surgery. Amniotic membrane contains large number of effective and reproductive cells and growth factors. To investigate the possibility of using this amniotic graft for wound healing, dried and sterilized by gamma irradiation HAM, that declared as AmnioGraft, were prepared in the preparation unit, radiation technology department, Syrian atomic energy commission (SAEC), according to the published regulations, that recommended by international committee and established by the developed countries. The wide spread and deep degree of wound healing process on post-operation was conducted in the burn and plastic surgery ward of Damascus hospital, by covering the damaged area by AmnioGraft, often folded foure times was sutered on mean postoperative days 0, 3, 6, 9, and 12. Operation was performed using only AmnioGraft without upper layer of skinfold. The clinical and laboratory results indicated that, the use of AmnioGraft for chronic wounds can be considered highly effective to improve the healing of wounds. The pine, exudation/drynedd degree, weight blood cells (WBCs), and the body temperature were reduced, and the wound healing was accelerated. Based on this information the use of AmnioGraft for wound can be considered as regeneration graft without using upper layer of skinfold. Our recommendation to established surgery protocol for wound thealing by using AmnioGraft without without using upper layer of skinfold for applying its in the domestical hospitals.