تحضير أربع بُنى مأشوبة باستعمال حامل pLEXSY والجين المُرمزة لعامل النمو الشبيه بالإنسولين من النمط الأول IGF-I

Abstract

Insulin-like growth factor IGF1, a small protein produced by the liver, regulates many cellular events that ensure the correct growth of the body's organs and the body in general. Symptoms resulting from a deficiency of this protein vary from growth disorders to dwarfism. All of these symptoms are treated with replacement therapy by giving patients a recombinant form of IGF1, which is usually produced using bacteria. Despite the widespread use of bacterial-based protein production systems, they have several disadvantages. A new system for producing recombinant proteins overcomes most of these disadvantages. It is the LEXSY system, which relies on using Leishmania instead of bacteria to produce proteins. Leishmania is characterized by producing proteins in shapes similar to natural ones through its ability to properly fold the protein and to carry out post-translational modifications. In this work, the pLEXSY vector was used to clone the gene encoding human IGF1 in two forms, either alone or combined with the green fluorescent protein GFP, and in two ways, the first of which ensures its production within cells while the second ensures its secretion into the culture

medium. This is the first time that these structures have been produced with the aim of recombinant protein production in a new system that overcomes the difficulties of producing it in bacteria.