Optimization Of Origanum vulgare L. Micropropagation and It Is Effect On Oil Content.

Abstract

This research was achieved to obtain a protocol for micropropagation of Origanum vulgare through cultivation of single nodal segment on MS supplemented with different concentrations of BAP (6-Benzyl Amino Purine) (0,0.5,1,1.5,2,2.5,3) mg/L+ NAA (Naphtalene Acetic Acid)(0.1) mg/L and rooting on 1/2MS supplemented with different concentrations of NAA (0.25,0.5,1,1.5,2,3) mg/l.

Results indicated that the most effective medium for shoot formation and growth is MS medium supplemented with 2 mg/L BAP+ 0.1 mg/L NAA at maximum number of shoots 4.60,and shoots length 2.23 cm.

while the best rooting rate (72.5%) was obtained on 1/2 MS medium containing 1mg/L NAA with maximum number of roots 8.05,and root length 4.95 cm,and thickness 2.43 mm . Micropropagated plants were acclimatized and transferred to soil with success. The essential oils were extracted from shoots of wild plant and field grown plant  and  also from in vitro plants to study their components by GC-MS. The amount of oil was higher in field grown plants (1.2% ) compared with wild plants which contain(0.4% ) oil, Terpinen-4-ol was the dominant component in the oil of wild plants with amount (24.90%) and (27.69%) in harden plants.