Evaluation the ability of the Calcium hydroxide Plus Points in eliminating Enterococcus faecalis in the roots canals(in vitro study)

Abstract

Aim of the study: This study aims at compare between calcium hydroxide- saline paste and calcium hydroxide plus points (Colten) - saline in eliminating Enterococcus faecalis bacteria within the infected root canals.

Materials and Methods: 30 canals of human single-rooted and single-rooted first premolar extracted for orthodontic reasons were prepared after cutting their crowns in order to standardize the length of the roots to 17 mm, then they were randomly distributed to two equal groups, the first group (n = 15) calcium hydroxide plus points- saline, group II (n=15) calcium hydroxide 1 g - Saline 1.5 ml.

Then the root canals were contaminated with the prepared bacterial suspension and the sample was incubated in the incubator under air conditions at 37°C for 7 days, then the bacterial units were counted before applying the drug, then the samples were filled with the appropriate substance and incubated in the incubator at a temperature of 37 degrees Celsius for 7 days, then the bacterial units were counted, and the number of bacteria was compared between the two substances applied to the samples. The data were statistically analized using SPSS using mann –whitney test.

Results: It was found that there was a statistically significant difference at the level of significance (0.05) in the results of the census of Enterococcus faecalis between the two groups of teeth studied after  using the dressings. The number of germs compared to the number of germs in the group of teeth to which calcium hydroxide plus points-saline was applied.

Conclusion: The calcium hydroxide-saline plaster had a significant effect on decreasing the bacterial population of Enterococcus faecalis compared to the calcium hydroxide plus points -saline dressing.