Evaluation of the Photodynamic Therapy in The Treatment of Oral Candidiasis

Abstract

 Abstract:

Aim of the study:  This research aimed was to evaluate the effective of  photodynamic therapy PDT   with methylene blue MB  using Diode laser  in the treatment of Oral Candidiasis.

Materials and methods: The study sample consists of 11 patients (7males and 4 females) selected from the oral medicine clinic at Faculty of Dentistry, Damascus University, and the participants with clinical and microbiological diagnoses of type  Oral Candidiasis received six sessions of PDT with MB (450µg/mL), and after 10 min of incubation, were illuminated by light emitting-diode light at 650 nm   three times a week for 20 days. Mycological cultures taken from the infected area and standard photographs of the cases were taken at baseline (day 0), at the end of the treatment  and at the follow-up time intervals (days  15 , 30 and 60). The colony-forming units (CFU ) were  seeded and quantified on Sabouraud agar tubes. Data were analysed by paired-samples T test.                                                    

Results: The treatment significantly reduced the CFU  at the end of the treatments and on )15, 30, and 60 day) of the follow-up period. Also the PDT  showed clinical success to and decrease the oral lesion and clinical symptoms for the patients at the end of the treatments and on day 30 and 60 of the follow-up period.  

Conclusions: This clinical study suggests that PDI can reduce fungal load and decrease the inflammation degree in patients with Oral Candidiasis.

Keywords: Candida, Oral Candidiasis, methylene blue,

photodynamic therapy, diode laser .