Improving the Production of Bio-Nanofibers Using Rotary Electro-Spinning Technology

Abstract

In this paper, nonwoven webs were formed using the principle of rotary Electrospinning (needleless), which is based on the use of electrostatic force

to form fibers from polymer solutions with diameters ranging from 10 nm to a few microns. The production of nanofibers with geometric arrangement and shape requires precise control of the electric field values in addition to the ability to guide the fibers during the electrospinning process, guides and collectors were used to develop the deposition of nanofibers and obtain nanowebs with greater durability and regular porosity. The samples were analyzed and the results showed the formation of membranes with nanofibers with diameters ranging from (100-300 nanometers) arranged in several layers, in addition to the production of nanofibers webs with oriented fibers for use in restoration operations in the medical field and in the field of cell transplantation.

To detect bacterial growth, urine samples were taken from several patients and cultured on nanowebs to aid in medical diagnosis of the type of bacterial infection occurring, and to confirm the type of germ and treat it. the test was conducted in three media: (EMB) medium, which grows gram-negative bacteria, MacConkey agar medium, which grows gram-negative bacteria, and blood glue medium, growing Gram-positive bacteria, and the results showed the presence of E-Coli bacteria in the MacConkey agar medium.

On the other hand, when comparing the growth of bacterial colonies in the media containing the nanowebs with the media that did not contain it, it was found that growth in the media containing the nanowebs was dense (thick) and is very clear.