Extraction of Catichol Oxidase from Oak (Quercus calliprinos L.) and Determining the optimal conditions for it's activity

Abstract

In this research the enzyme catechol oxidase was extracted from some organs of Quercus calliprinos L.. Samples were taken from Jableh and swaida. there were morphological differences  between Jableh samples  , so they were grouped in to  Jableh(1) and Jableh(2).

The enzyme was extracted from young and old leaves and from the fruits, after that the activity and the optimum conditions, especially, temperature and pH were evaluated.

Results showed that the highest activity of enzyme was in young leaves then in the old ones followed  in fruits, there was a difference in pH between studied samples, the optimum pH for enzyme activity  in Jableh 1 Quercus  was pH= 5.5 (1.614U) , whereas , the optimum pH for enzymes in Jableh 2 and swaida Quercus  was at pH =6.5(1.440, 1.046U) respectively, the optimum temperature for enzyme activity was at 25ºC for all samples.

The results indicate that there was a difference in the activity according to the source of the plant sample and the developmental stage/age, it was found that the activity was significantly higher in all cases in the samples of Jableh (1) followed by Jableh (2) and Swaida (2).