Effect of Nutrient Media Enhanced with Plant Growth Regulators on Rooting of In-vitro Vegetative Progenies of Digitalis Purpurea L
Keywords:
Rooting, Digitalis Purpurea, vegetative strains, nutrient media, plant growth regulatorsAbstract
This research aimed to study the effect of nutrient media and plant growth regulators on rooting of in-vitro vegetative strains of Digitalis Purpurea.
Experiments were carried out by a gradient series of concentrations of a group of auxins (Dichlorophenoxy acetic acid 2,4-D, indole acetic acid IAA and naphthalene acetic acid NAA) and cytokinins (benzyl aminopurine BAP, kinetin and benzyl adenine BA) and Gibberellic acid GA3 in four nutrient media (MS, LS, B5 and 5C01) to select the optimal media and the optimal concentration.
The results showed a variation in root induction percentage, depending on the nutrient medium and the type and concentration of the growth regulator.
The B5 medium enhanced with IAA was significantly superior in comparison with the studied media, and the two vegetative strains DPM1 and DPM3 achieved significant superiority with 90% induction percentage when treated with 0.5 mg.L1- of IAA in B5 medium. Whereas, the results showed a significant superiority of the MS medium enhanced with NAA compared to the studied media, and the DPM1 strain treated with 0.5 mg.L 1-of NAA in the MS medium achieved a significant superiority with a root induction rate of 85% compared to the studied vegetative strains in the nutrient media. Moreover, the DPM3 vegetative strain treated with 0.5 mg.L1- of gibberellin acid GA3 added to the B5 medium was significantly superior with a 90% induction rate compared to the studied strains in different media. In general, the variation in root induction rates was due to the different components of the nutrient medium, the type and concentration of growth regulators, and the mechanism of their physiological effect.
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