Isolation and culture of skin Fibroblast from skin of the adult Rats (Wister rat)

Abstract

Researchers are interested in culture and storage of stem cells, whether embryonic or adult, for scientific and therapeutic purposes. The fibroblast are the origin of the mesenchymal cells, the fibroblast used in cellular therapies, because they help in wound healing and they have a high regeneration capacity.

Fibroblasts were isolated from the skin of adult rats (20 adult male rats), and cultured in DMEM and 15% FBS medium in a gelatin-coated culture box in a CO2 incubator under the conditions (5% co2-temperature 37°- humidity (75°C).

Were studied under inverted microscope, it was observed that cells with many elongations and branches showed clear adhesion to the bottom of the culture flask. The culture medium appeared transparent without any turbidity or bacterial or fungal contamination, and the medium maintained the pink color.

Through the experiment, the most conditions with making daily and continuous efforts to keep these cells healthy and protected so that they can reproduce easily.