Regulation of proteolipid protein (PLP) expression in oligodendrocytes cell lines of animal models for Pelizaeus-Merzbacher Disease (PMD) using RNA interference (RNAi) technology

Authors

  • Dr. Zeina Malek

Keywords:

RNA Interference, Myelin sheath, Oligodendrocytes

Abstract

In this study, RNA interference technology was used to inhibit gene expression of the Proteolipid Protein (PLP) on Oligodendrocytes cell lines from Jimpy mutant mice, an animal model for Pelizaeus-Merzbacher Disease (PMD), which is a X-linked sever leukodystrophy. The preliminary findings demonstrate a significant correction of functional aspects in oligodendrocytes, ie effect of dibutyryladenosine 3 ,5 - cyclic monophosphate (dbcAMP) application on both Myelin Basic Protein and Carbonic Anhydrase II expression. Our results suggest a breakthrough in leukodystrophy therapy especially if coupled with effective delivery system in vivo.

 

 

 

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Published

2021-08-26

How to Cite

Regulation of proteolipid protein (PLP) expression in oligodendrocytes cell lines of animal models for Pelizaeus-Merzbacher Disease (PMD) using RNA interference (RNAi) technology. (2021). Damascus University Journal for the Basic Sciences, 34(1). https://journal.damascusuniversity.edu.sy/index.php/basj/article/view/1241