Contribution in determining the degree of taxonomic relationship of five species of Euphorbia L. (Euphorbiaceae) Depending on Protein Contents Properties

Abstract

The protein content in the leavesand in the seeds of electrophoresis (SDS-PAGE) in five Species of genus Euphorbia found in Syrian flora was determined for 5 species of Genus Euphorbia L. found in the Syrian flora: E. helioscopia, E. peplus, E. prostrata, E. pilulifera, E. paralias.

In order to reveal the usefulness of these criteria in determining the degree of taxonomic relationship between these species.

Quantitative protein analysis in the studied species showed different percentages, the lowest percentage of E. paralias (2.75%), and the highest protein found in E. peplus (19.06%).

The resultes helped us to obtained a pylogenetic tree by conducting cluster analysis

of the results obtained from the protein footprint of the seeds of studies species, and 40 samples were used for this analysis.

The first pair of species was separated into two clusters: the first included E. paralias and the other two species were joined by a similarity of 0.065 and separated under the second cluster into two clusters. The first species E. helioscopia and  E. peplus were associated with a similarity of 0.143, and the two types               E. prostrate and E pilulifera by a similarity of 0.333.