Determination of cyclodextrin glycosyltransferase activity (CGTase) produced by Bacillus spp. isolated from Syrian soils

Abstract

ABSTRACT:

Fifty soil samples were collected from different areas of Damascus city and its suburb. They were suspended in Horikoshi II selective medium. Bacterial was isolated from 24 soil samples and 56 isolates were obtained and purified for primary screening and detection for their efficiency in cyclodextrin glycosyltransferase (CGTase) production which ranged between 1.1-4. Five isolates were chosen accordingly (15-1, 25-7, 26-7, 46-1, 50-1) and the secondary screening was performed to estimate the enzyme activity of the five chosen isolates every 24 hours for 3 days at 30°C to determine the highest isolate efficiency in β- Cyclodextrin (β-CD) production. Results showed that isolate (25-7) revealed the highest activity in enzyme production of 26.29 U/ml after 24 hours of incubation, while isolate (46-1) revealed the lowest activity of 2.16 U/ml after 72 hours. The most efficient isolate (25-7) was identified as Bacillus licheniformis.

Keyword: Cyclodextrin Glycosyltransferase Enzyme, Cgtase,

Cyclodextrins, Β-Cd, Β-Cyclodextrin, Enzyme Activity, Horikoshi Ii, Bacillus Licheniformis.