Chemical analysis of essential oil of Lavandula officinalis L. planted in Nabek, Syria, and its effect on Xanthomonas translucens bacteria.

Abstract

Flowers of Lavandula officinalis L. were collected from cultivated plant Lavandula officinalis L., hydro-distillation was used to extract essential oil from fresh flowers, the oil yield was  2.7 % (v/w),  the chemical analyses using GC/MS, showed that 20 compounds were identified represent  98.37 % of total oil, the main components were  Linalool (51.03%) and Camphor (14.92%), four compounds were recorded for the first time: Linalyl anthranilate, Linalyl iso-valerate, Butanoicacid and β-Cubebene (3.64, 0.62, 0.44, 0.39 % respectively). The results of phytotoxicity experiments showed that the concentration of 10 µl/ml was the highest concentration of essential oil, which didn’t have a negative effect on wheat seed germination and seedlings growth. On other hand, the bioactivity was investigated, the minimal inhibitory concentration of the oil against two isolates of Xanthomonas translucens (Xt10، Xt4.2) were determined to be 0.75- 1 µl/ml respectively.

The effect of lavender oil on population size of X. translucens on wheat seeds indicated that 5 µl/ml was the lowest concentration reduced the bacterial count about 100 times, and improved the wheat seed germination and seedling growth.