First record of the bacterial communication signal 11-methyldodecenoic acid from the DSF family in the angular spot bacteria on the cotton, Xanthomonas citri subsp. malvacearum

Abstract

Xanthomonas citri subsp. malvacearum (Xcm), which belongs to the genus

Xanthomonas, is one of the most dangerous pathogens that effect to the cotton plant, as it infects most of its parts, causing quantitative and qualitative economic losses. The Xanthomonas sp. use bacterial communication system Quorum Sensing (QS), which is mediated by Diffusible Signal Factor (DSF-family), signals to regulate and coordinate the expression of pathogenicity genes in response to their numerical density in the medium. Therefore, the research aimed to detect bacterial signals belonging to the DSF family in Xcm bacteria using the liquid chromatography method associated with a liquid chromatography-mass spectrum (LC-MS). The results of LC-MS analysis revealed a number of DSF family molecules Cis-2-undecenoic acid, 12-methyltetradecenoic acid, and 11-methyldodecanoic acid, with ionic weights 197, 241, 213 z\m, respectively. It should be noted that this research has shown, for the first time, the presence of one of the DSF derivatives, which is 11-methyldodecanoic acid, in one of the species of Xanthomonas.