The induction effect of abnormal cellular growth to p- Phenylenediamine preparations

Abstract

ρ-Phenylenediamine (PPD) is a common primary intermediate used in the composition of permanent hair dyes. The harmful effects which are caused by this type of hair dyes are directed towered further studies to evaluate the safety. The study is applied to five different formulas of hair dyes containing ρ-Phenylenediamine as basic primary intermediate. These hair dyes were applied on HEK293T cell line after preparing their solutions by Franz cell. The cell line was treated with four concentrations of hair dye solutions and incubated for 24 hours in 5% CO2 incubator at 37ºC then tested by MTT assay. The results appeared significant increase in viability of cells after added four formulas of hair dyes whereas the increase of viability of cells that caused by the fifth one was not significant compared to control. Therefor the hair dyes containing ρ-Phenylenediamine might be activator to cell growth up to normal in vitro according to its composition of it and safety of components.

Key Words: Ρ-Phenylenediamine, Franz Cell, HEK293T, MTT Assay.