New Spectrophotometric Method for the Determination of Tamsulosin Hydrochloride in Bulk and Pharmaceutical Dosage Forms

Abstract

A new, simple, sensitive, accurate, selective  method  has been developed for the determination of  Tamsulosin hydrochloride  in bulk  and in pharmaceutical dosage forms. The developed  method  is a chemical derivatization method involving proton transfer from the 2,4,6-trinitrophenol ( picric acid) to the secondary amino group of Tamsulosin   in acetonitrile as a solvent  to form a yellow charge transfer complex exhibiting maximum absorption (λ _max) at 400 nm. The method was linear in the concentration range [1-12  µg/ml] with correlation coefficient (R²= 0.9979), intra-day, inter-day precision (as RSD)and accuracy were determined. The obtained results were statistically validated .The method was successfully applied to the determination of  Tamsulosin hydrochloride  in tablets. The percentage recovery was 102.3 % ±  0.591 (n=5).The developed method is considered to be simple, accurate and specific for Tamsulosin HCl determination as raw material or in tablets.

Key words: Tamsulosin Hydrochloride,  Picric Acid, Chemical Derivatization, Charge Transfer  Reaction, Spectrophotometer.