Isolation, purification and in vitro multiplication of Haematococcus pluvialis using a photobioreactor and determination of its growth phases

Abstract

Algae samples were collected from a pool of stagnant water belonging to Barada River in Al-Rabwa area in Damascus city. Haematococcus pluvialis was isolated and purified in vitro under sterile conditions. This alga was then grown in BBM in the photobioreactor in batches, to obtain a suitable biomass, and the culture conditions were determined (temperature 25°C, illumination intensity 10,000 lux, 12light/12dark system, continuous aeration 2 L/min, pH 5.5 - 6.5 for 18 days, the growth phases and growth rate of this alga were determined from the daily optical absorbance values, at wavelength 680 nm, as the logarithmic phase started on the fifth day and its end on the twelfth day.