Effect of nutrient media enhanced with plant growth regulators on the variation in the mineral elements content of Digitalis purpurea L callus cell lines subcultures

Abstract

This research aimed to study the effect of the nutrient media enhanced with plant growth regulators on the variation of the mineral content in the subcultures of the selected cell lines of Digitalis purpurea callus.

Selected DPM1, DPM2, DPM3 and DPM4 cell lines from Digitalis purpurea callus were cultured in MS, B5, LS and 5C01 media, and re-cultured on the same media and concentrations until the subculture 80. Cell cultures (subculture10, subculture20, subculture40 and sub-culture80) were selected from each studied cell line as an indicator to study the elements contents, and with the presence of seed and in vitro plant samples as two controls.

The studied cell lines showed a variation in the accumulation of the studied mineral elements at the level of different media, and at the level of subcultures. The iron content decreased in the cell subcultures in all nutrient media compared to the control, and the zinc contents varied with the superiority of the subculture80 of the DPM1 and DPM3 cell lines in the media MS and LS, where it exceeded 54 ppm. Moreover, copper and magnesium contents varied within the media, cell line and cell subcultures, and the DPM1 cell line grown in MS medium excelled in all its subcultures in the accumulation of sodium and potassium contents, while the DPM4 cell line grown in 5C01 medium was superior in the accumulation of calcium content. On the other hand, the lead content decreased in the cell cultures and was within the permissible limits in all the cell subcultures of the studied cell

lines and the two controls.