A rapid and valid HPLC method for the simultaneous determination of methanol, formaldehyde and formic acid in their mixtures without derivatization

Abstract

A new simple and reliable HPLC method is described and validated for the simultaneous determination of methanol, formaldehyde and formic acid in their mixture without derivatization. The method was validated in terms of its specificity, linearity, accuracy, precision, and limits of detection and quantification. The analysis cover concentration ranges: 0.00322 - 0.0516 M for formaldehyde, 0.0065-0.1051 M for formic acid, and 0.0067- 0.1085 M for methanol. The method uses a cation exchanger column (Eurokat)[1], with a 0.25 ml/min flow rate of 0.011 M sulfuric acid as a mobile phase in the presence of refractive index (RI) detector.

[1] Knauer Eurokat, H+ Form 300 x 800mm ID