Determination of the quantitative content of some bioactive chemical compounds in Spirogyra fluviatilis
Keywords:
Spirogyra fluviatilis, Culture, Phenols, AntioxidantsAbstract
Spirogyra fluviatilis was isolated from Lake Tishreen 16 in Lattakia and morphologically classified according to the number of plasts and the shape of the fertilized egg and then grown in open ponds with the addition of appropriate nutrients. Ash, extraction yield, pigment quantity, nutritional value, phenol quantity and antioxidant activity were measured. The ash content was 119 ± 1.4 mg/g and 55 ± 0.2 mg/g of methanol extract. The amount of chlorophyll a and b and carotene were 4.19 ± 0.4, 1.77 ± 0.2, 1.22 ± 0.04 mg/g, respectively, of the dry weight of the moss. The nutritional
value of the algae was tested and the values of protein, sugars and fats were recorded at 307.57 ± 0.87, 204.4 ± 1.29, 45.5 ± 0.31 mg/g, respectively. The values of the total content of phenols and flavonoids in methanol extract were shown at 420.6 ± 0.017, 204.3 ± 2.92, respectively. Methanol extract showed higher antioxidant activity than chloroform extract, scavenging 50% of free radicals in Dpph solution at a concentration of 82.39 mg/L of methanol extract. The results of this study support the use of Spirogyra as a dietary supplement and an important source of natural colorants and antioxidants.