Isolation of Escherichia coli from effluents of some Damascus University hospitals and determination of their susceptibility to Beta lactams Antibiotics

Abstract

A survey on several Damascus university hospitals was carried out to determine the presence of E.coli resistant to beta lactams antibiotic and to determine the percentage of isolates that secreted Beta-lactamase in the liquid effluents of these hospitals. The number of isolates was 150 for  E. coli.

Sensitivity tests using a number of Beta Lactam antibiotics belonging to the group of penicillins and cephalosporins of the first and second and third generation were conducted. The percentage of resistance to first generation of cefalosporin group of Cefadroxil (CFR), Cefazolin (CZ), Cephalexin (CL), and third generation Cefapodoxime (CDP) reached 100%, while the percentage of resistance towards Cefaclor (CFC) from the cephalosporin second generation reached 86%, and it reached 93% toward Cefatoxime (CTX),and 80 % toward Cefaperazone (CPZ), and%78 towards Cefteriaxon (CTR), all belonging to the third generation cephalosporins.As for the resistance of E.coli isolates towards penicillins they reached 93% towards Amoxycillin, while 42% of the isolates tolerated high concentration of 500µg/ml of Ceftriaxone.Results also showed that 88% of the resistant isolates produced Beta-lactamase.