Preparation of the LMJF-31-1450 gene that encoding PSA protein of Leishmania major as DNA vaccine

Abstract

Leishmaniasis remains a major health problem in many countries worldwide. Although the production of new drugs is promising, the development of safe and effective vaccines remains the best hope, especially given the emergence of drug resistance. One of the main strategies to control leishmaniasis is to develop a DNA vaccine. In this regard, various antigens have been evaluated as parts of potential DNA or recombinant vaccines against leishmaniasis. Here, we expressed a recombinant eukaryotic plasmid containing gene that expressed of PSA protein in its secreted form from Leishmania major promastigotes and PSA protein fusion with superfolder green fluorescent protein (sGFP) for enhancing PSA folding and improve its stability In addition to using sGFP as a marker for the expression. We succeeded in confirming the validity of the produced plasmid structures and verified the production of the fusion protein in HEK-293T cells using Western blotting. Thus, we have obtained the basic material for a DNA vaccine whose protective role can be tested in animal models.