Detection of the plasmid-borne of Extended Spectrum Beta-Lactamases in E. coli isolates from some Damascus University's hospital wastewater
Keywords:
Beta-lactamase, ESBLS, plasmid curing, hospital waste water.Abstract
The type of beta lactamases was determined in Escherichia coli isolates from
waste water of some of Damascus University's hospitals. Plasmids were extracted using the boiling method and the alkaline method. Results showed that boiling method is the most convenient method for plasmid extraction. Plasmid curing was carried out by applying two methods; the first was by incubation at 42 Cº and subculturing 8-10 times, the second method was by adding SDS concentration of 1.25%, 2.5% and 5%. The best curing result was obtained using the subculturing method at 42Co.
Two types of enzyme inhibitors (Clavulanic acid and sulbactam) were used to determine the type of the beta lactamase produced by the studied isolates. DNA primers used in this study belonged to two different groups (SHV and CTX) to determine the group to which enzymes belong. Results obtained showed that ESBLS of class A belonging to group CTX appeared to be most frequent among the studied isolates
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