Molecular characterization of some potato (Solanum tuberosum) genotypes using Inter Simple Sequence Repeat (ISSR)

Abstract

Ten potato genotypes grown in Syria were studied for Molecular characterization to benefit from the development of effective breeding programs of potato. 16 ISSR primers were used for molecular characterization; all used primers produced DNA fragments in all studied genotypes.The total number of amplified bands was 436 bands with an average of 27.25bands/primer. The total number of polymorphic bands was433with and average of 27.06bands/primer.Only3monomorphic bands were detected. The average of polymorphism percentages for the used primers was 99.30%. The closely relatedgenotypes were Tarus and Falukawith a genetic distance value of 0.523, while the most distant genotypes were Challenger and Soraya with a genetic distance of 0.836. The cluster analysis, based on ISSR data, divided the studied genotypes into 5 groups,the first group included three genotypes: Rumba, Tarus and Faluka, the second group included three genotypes: Camberra, Alaska and Soraya, the third group included Challenger and Kastelli, the fourth group included Royal only, and the fifth group included Sylvana. These results indicate that the ISSR is an effective tool to determine the genetic variation among different potato genotypes, and the high genetic variation between the analysed genotypes indicate also the possibility of using them in the potato genetic improvement programs.